cd34 specific antibody  (Bioss)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel cryoprecipitate-enriched PRP (Cryo-PRP) gel with enhanced mechanical strength and regenerative capacity accelerates enterocutaneous fistula healing

doi: 10.3389/fbioe.2025.1668608

Figure Lengend Snippet: Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of CD34 (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.

Article Snippet: Subsequently, the sections were incubated overnight at 4 °C with rabbit anti-CD34 antibody (BIOSS, bs-064R; 1:100 dilution).

Techniques: Immunofluorescence, Immunohistochemical staining, Staining, Control, Expressing

Journal: Frontiers in Pharmacology

Article Title: Mangiferin mitigates dexamethasone-induced insulin resistance in rats: insight into vascular dysfunction and hepatic steatosis

doi: 10.3389/fphar.2025.1572758

Figure Lengend Snippet: Impact of Mang on the aortic expression of CD34 and VEGF: Panel (I) : Photomicrographs of CD34-immune stained sections in the aorta: (A) CTRL group: showing strong positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads). (B,C) DEX-treated group: showing weak positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads) compared to the CTRL group. (D) Mang (25 mg/kg)-pretreated group: showing mild positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads) but more than the DEX-treated group. (E) Mang (50 mg/kg)-pretreated group: showing moderate positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads). (F) Mang (100 mg/kg)-pretreated group: showing strong positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads) more or less similar to the CTRL group. Panel (II) : Photomicrographs of VEGF-stained sections in the aorta: (A) CTRL group: showing minimal VEGF immunostaining in the cytoplasm of endothelial cells (arrowhead). (B,C) DEX-treated group: showing more abundant positive VEGF immunostaining in the cytoplasm of endothelial cells (arrowheads) and in vascular smooth muscles (curved arrows) more than CTRL group. (D) Mang (25 mg/kg)-pretreated group: showing strong positive VEGF immunostaining in the cytoplasm of endothelial cells (arrowheads) and in vascular smooth muscles (curved arrows) but less than the DEX-treated group. (E) Mang (50 mg/kg)-pretreated group: showing moderate positive VEGF immunostaining confined to the cytoplasm of endothelial cells (arrowheads). (F) Mang (100 mg/kg)-pretreated group: showing scarce positive VEGF immunostaining confined to the cytoplasm of endothelial cells (arrowheads) more or less similar to the CTRL group. Image magnification = ×400. CTRL: control; DEX: dexamethasone; Mang: mangiferin; VEGF: vascular endothelial growth factor.

Article Snippet: Polyclonal antibodies were obtained from Thermo-Fisher Scientific Anatomical Pathology, California, United States, including TNF-α; Cat No. PA1-40281, IRS1; PA5-104876, eNOS; PA1-037, CD34; MA5-35202, respectively, and VEGF antibodies (Cat No. bs-0279R, Bioss, Inc., Woburn, Massachusetts, United States) for utilization in the Biotin-Avidin complex method ( ).

Techniques: Expressing, Staining, Immunostaining, Muscles, Control

Journal: Frontiers in Pharmacology

Article Title: Mangiferin mitigates dexamethasone-induced insulin resistance in rats: insight into vascular dysfunction and hepatic steatosis

doi: 10.3389/fphar.2025.1572758

Figure Lengend Snippet: Schematic illustration of the protective mechanism of Mang against DEX-induced IR in rats. AKT: protein kinase B; ALT: alanine aminotransferase; AMPK: AMP-activated protein kinase protein kinase; AST: aspartate aminotransferase; DEX: dexamethasone; eNOS: endothelial nitric oxide synthase; EPCs: endothelial progenitor cells; ET-1: endothelin-1; FFA: free fatty acids; FOXO-1: forkhead box protein O1; FSI: fasting serum insulin; G6Pase: glucose 6-phosphatase; GS: glycogen synthase; GSH: reduced glutathione; GSK-3 glycogen synthase kinase 3; HDL-C: high-density density lipoprotein; IRS1: insulin receptor substrate-1; JNK: C-Jun N-terminal kinases; LDH: lactate dehydrogenase; LDL-C: low-density density lipoprotein; Mang: mangiferin; MDA: malondialdehyde; NF-κB: nuclear factor-kappa B; NLRP3: NOD-like receptor family pyrin domain-containing 3; OGTT: oral glucose tolerance test; PEPCK: phospho-enol pyruvate carboxy kinase; PGI2: prostacyclin; PPAR-γ: peroxisome proliferator-activated receptor-γ; ROS: reactive oxygen species; SOD: superoxide dismutase; TC: total cholesterol; TG: triglycerides; TNF-α: tumor necrosis factor alpha; VCAM: vascular cell adhesion molecule; VEGF: vascular endothelial growth factor; VLDL-C: very-low-density lipoprotein.

Article Snippet: Polyclonal antibodies were obtained from Thermo-Fisher Scientific Anatomical Pathology, California, United States, including TNF-α; Cat No. PA1-40281, IRS1; PA5-104876, eNOS; PA1-037, CD34; MA5-35202, respectively, and VEGF antibodies (Cat No. bs-0279R, Bioss, Inc., Woburn, Massachusetts, United States) for utilization in the Biotin-Avidin complex method ( ).

Techniques: